Application Oriented is the Core of Traditional Chinese Medicine Research / 世界科学技术-中医药现代化

Traditional Chinese Medicine (TCM) has distinctive traditional features and unique therapeutic advantages.The characteristics of TCM including long history, rich theory, efficacy diversity, complex chemical composition, fuzzy mechanism, leading health care, compound-based, dose confidential, and dialectical pharmacy and so on, but TCM research findings with few practical applications now. We need a profound understanding of the characteristics of TCM and Chinese medicine research and application of full advantage of clear objectives that to accurate selection of TCM research focus and appropriate methods, creative design scheme of TCM. In this way, we may have satisfied the anticipated target about TCM and TCM academic truly promote the maintenance of health. In the medicine industry is bound to promote the great development of TCM to the world.

To Study the Analgesic, Anti-diarrhea and Anti-inflammatory Effects of Wulian Compound Extract / 浙江中医药大学学报

[Objective] To study the analgesic, anti-diarrhea and anti-inflammatory effects of Wulian compound extract. [Method] To study the analgesic effect of Wulian compound extract on mice with hot-plate test, writhing test, and formaldehyde stimulation. To study the anti-inflammatory effect of Wulian compound extract by mice ear-swol en induced by xylene and abdominal cavity capil ary Permeability text .To study the anti-diarrhea effect of the Wulian compound extract with the method of diarrhea induced by castor oil and senna. [Results] Wulian compound extract can inhibit the pain caused by high fever, acetic acid and formaldehyde. Inhibit the xylene induced ear swel ing of mice and increase of celiac capil ary permeability induced by acetic acid. At the same time Wulian compound extract can prolong diarrhea latency induced by the senna and castor oil, reduce the frequency of diarrhea. [Conclusion]Compound Wulian extracts have significant antidiarrheal effect, anti-inflammatory, analgesic.

Review on the Research of the Components, Pharmacological Actions and Toxicity of Polygonum Multiflorum Thunb(Heshouwu) / 浙江中医药大学学报

[Objective] To sum up researches on components, pharmacological actions and toxicity of Polygonum multiflorum Thunb(Heshouwu). [Method] The research reference about Heshouwu in CNKI, VIP and PUBMAD database is col ected and calssified to three aspects on components, pharmacological actions and toxicity.[Result]①On components:Heshouwu contains stilbene, lecithin, anthraquinone, flavonoids, tannin and trace elements. The stilbene and anthraquinone are the mainly active components. Producing area, growth years, harvest time and processing methods are factors affecting the content of the components such as TSG in Heshouwu; Macroporous adsorption resin is the main separation method for preconcentration of TSG and anthraquinone constituents; ② On pharmacological actions and toxicity: Heshouwu has effects of hypolipidemic and liver protection, antioxidative, anti-osteoporosi, hpyerglycemic, antidepressive and antibiosis, the hypolipidemic and liver protection are the main effects which have been studied more. A reversible liver injury which displays as abnormal liver function parameter and bile metabolic disorder would be induced if the Heshouwu is administrated with a large dosage for long term. The possible components which would induce the toxicity in Heshouwu are tannin and anthraquinone, and the anthraquinone is the component with double effects of toxicity and therapy which should be considered in clinical application. [Conclusion]Reducing lipid and protecting liver is the main pharmaceutical function of the herb. As a commonly used Chinese herb for liver protection, the studies on alcoholic hepatic injury protection of Heshouwu may be a point for the researchers to focus on. The comparison studies on hypolipidemic and liver protection between Hesouwu and its processing products, and the correlations between components and pharmacological actions are worth study further.

Bioluminescent Monitoring the Influence of Cepharanthine on Colon Cancer Growth in Mouse Xenograft Tumor Model / 浙江中医药大学学报

[Objective]To investigate the anti-proliferative effects of CEP on HCT116 cells and in mouse xenograft model. [Methods]The in vivo anti-cancer activity of CEP was determined with Xenogen bioluminescence imaging in a xenograft tumor model. The cel-based multiple signaling pathway reporter assays were carried out to determine the effects of CEP on these pathways. [Results] CEP inhibited growth of human cancer cells, the IC 50 was 0.8~11.5 μM. CEP induced cellcycle arrest in S and G2/M phase. CEP also inhibited xenograft tumor growth in athymic nude mice bearing HCT116 cells. The xenograft tumor size was significantly reduced upon the treatment with CEP(10 or 20 mg·kg-1 body weight) for up to 3 weeks. Pathway-spe-cific reporter assays indicated that CEP effectively suppressed the NF-κB and MAPK/ERK signaling pathways. [Conclusions] Our results suggest that the anticancer activity of CEP in colon cancer cells may be mediated through targeting NF-κB and MAPK/ERK signaling pathways.

Comparative studies on scavenging DPPH free radicals activity of flavone C-glycosides from different parts of Dendrobium officinale / 中国中药杂志

<p><b>OBJECTIVE</b>To study the scavenging DPPH free radicals activity of flavone C-glycosides from different parts of Dendrobium officinale.</p><p><b>METHOD</b>The types and contents of flavonoids from different parts of D. officinale were analyzed by TLC and HPLC. The antioxidant effect was tested by scavenging DPPH free radicals activity.</p><p><b>RESULT</b>The stems, leaves and flowers contained the same type of flavone C-A glycosides and 8 common peaks were identified. The content of flavone C-A glycosides was significantly different. The content of flavone C-glycosides in leaves and flowers was higher than that in stems. The flavonoid in roots was less. Stems contained naringenin, which was not identified in root, leave and flower. Both stems and leaves had antioxidant capacity of eliminating DPPH free radicals, of which scavenging DPPH free radicals activity of leaves was better than stems.</p><p><b>CONCLUSION</b>Considering the content of flavonoid and antioxidant activity leave and flower of D. officinale may substitute stems. The study provides a preliminary basis for the development and utilization of leave and flower of D. officinale.</p>

Effects of three traditional Chinese medicine with pungent-flavor, warm-nature and meridian tropism in lung on lung-yang deficiency rats induced by compound factors / 中国中药杂志

<p><b>OBJECTIVE</b>To observe the effects of three traditional Chinese medicine (TCM) such as Amomi Fructus Rotundus, Perillae Folium and Angelicae Dahuricae Radix on lung-yang deficiency rats induced by compound factors.</p><p><b>METHOD</b>Lung-yang deficiency rats were established with three-factor combination, such as smoking (exogenous evil effect on lung), swimming in common and ice water (cold body and exhaustoin) and drinking ice water (inhale cold). Meanwhile, rats were given water extracts of the three TCM by intragastric administration for 24 days everyday. Indexes such as general behavior, weight, rectal temperature, back temperature and grip strength were observed. Blood was collected to determine NO, IgG in blood serum. Lung and heart were dissected to measure organs index.</p><p><b>RESULT</b>The water extracts of Amomi Fructrs Rotundus, Perillae Folium and Angelicae Dahuricae Radix could markedly heighten weight, back temperature, grip strength, content of IgG in blood serum, reduce content of NO in blood serum, lung index and heart index. The water extracts of Amomi Fructrs Rotundus and Perillae Folium could heighten rectal temperature.</p><p><b>CONCLUSION</b>Amomi Fructrs Rotundus, Perillae Folium and Angelicae Dahuricae Radix were TCM with pungent-flavor, warm-nature and meridian tropism in lung, which could improve the symptoms of physique emaciation, aversion to cold of the back, weary and acratia and so on. It provides an important reference for the regularity of the properties theories about pungent-flavor, warm-nature and meridian tropism in lung.</p>

Analysis of content changes of volatile oil and beta-elemene in oil in Sarcandrae / 中国中药杂志

<p><b>OBJECTIVE</b>To reveal the factors that affect the contents of volatile oil and beta-elemene in oil in Sarcandrae and to provide scientific basis for Sarcandrae's reasonable exploitation and quality assessment.</p><p><b>METHOD</b>A GC method was established and the contents of volatile oil and beta-elemene were determined in Sarcandrae from different length of time to grow, different medicament portions, different collection periods and different length of time to dry.</p><p><b>RESULT</b>The length of time to grow had no significant effect and the collection periods had effect on the volatile oil yield and content of beta-elemene. The volatile oil yield and content of beta-elemene were the highest in Sarcandrae harvested in December. The yield of volatile oil in different medicament portions descended in an order of roots, leaves and stems. The content of beta-elemene was the highest in leaves and the lowest in stems. With the increasing of the length of time to dry, the volatile oil yield and content of beta-elemene decreased calculated on an anhydrous basis.</p><p><b>CONCLUSION</b>The established method is simple, accurate and repeatable. It can be used for the quality control of beta-elemene in Sarcandrae. The study provides a valuable basis for the quality assessment, the development and utilization of Sarcandrae.</p>

Effect of Jimaitong tablet combined with nifedipine on blood-pressure and mechanisms investigation in spontaneously hypertensive rats / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effect and its mechanism of antihypertensive of drug combination of Jimaitong tablt and nifedipine on spontaneously hypertensive rats.</p><p><b>METHOD</b>The spontaneously hypertensive rats (SHR) were treated by intragastric administration (ig) with Jimaitong (450 mg x kg(-1)), Jimaitong (300 mg x kg(-1)) combined with the nifedipine (0.5 mg x kg(-1)), nifedipine (5 mg x kg(-1)) for 8 weeks. Blood pressure and heart rate was measured. The level of oxidation indicators, lipid indicators, as well as hormones related to blood pressure was detected.</p><p><b>RESULTS</b>Compared with that in the control group, systolic blood pressure, diastolic blood pressure, mean arterial pressure of SHR decreased significantly in all the three administration groups (P < 0.01). Heart rates of nifedipine group was faster than other groups (P < 0.05). In combination group, contents of URE, MDA, ET and Ang II were decreased significantly (P < 0.05, P < 0.05, P < 0.05, P < 0.01), the contents of NO and CAMP, SOD and NOS were significantly enhanced (P < 0.05).</p><p><b>CONCLUSION</b>Combination use of Jimaitong and nifedipine has synergistic effects on anti-hypertension in spontaneously hypertensive rats. It indicates the potential applications of combination of traditional Chinese medicine and western medicine for the hypertension treatment.</p>

Preparation of submicron emulsion of fresh Zhongjiefeng volatile oil / 中国中药杂志

<p><b>OBJECTIVE</b>To prepare the submicron emulsion of fresh Zhongjiefeng volatile oil.</p><p><b>METHOD</b>The Zhongjiefeng volatile oil submicron emulsion was obtained after passing the elementary emulsion through a high pressure homogenizer. The physical and chemical stability of the emulsion was evaluated with the stability parameter of centrifugation, appearance of emulsion and the pH. The formulation and processing factors were optimized by single factor reviewing and orthogonal experimental design.</p><p><b>RESULT</b>By controlling various processing factors and optimizing formulation, the stable submicron emulsion of Zhongjiefeng volatile oil was prepared. Its mean particle diameter was 164-169 nm with PDI 0.084-0.107 and Zeta electric potential was -40 mV.</p><p><b>CONCLUSION</b>The formulation and preparation technique of the emulsion is reasonable.</p>

Pharmacological Study on the Function of "Qi-promoting, Blood-breaking, Stagnation-removing and Pain-relieving" of Zedoray Rhizome / 国际中医中药杂志

Objective To discuss Zedoray Rhizome on the correlation between its traditional Chinese herbal nature(Property and Flavor,Cannel Tropism,Efficacy and so on)and its pharmacologic actions.Methods By searching for and summarizing the literatures on the nature and pharmacologic actions of Zedoray Rhizome recorded in CNKI,CBM,Wanfang and Vip Database from 1989 to 2007,analyzed the correlation between its herbal nature and its modern pharmacologic actions.Results The efficacies of"Qi-promoting Blood-breaking and stagnation-removing" are related with the modern pharmacologic actions of resisting tumor,improving factors of hemorheology,resisting inflammation and virus,and preventing acute liver injury,etc;the efficacy of "Warming middle energizer to relieve pain"means the function of improving activities of spleen,stomach and uterus in modern pharmacology;and the spleen channel tropism is related with and its pharmacologic action of enhancing immune activity.Conclusion There are some correlations between Zedoray Rhizome's traditional Chinese herbal nature and its modern pharmacologic actions.The correlations between Zedoray Rhizome's traditional Chinese herbal nature and its modern pharmacologic actions are waiting for the further improvements to provide the dependable basis for the clinical application.

Synergistic Effects and Decreasing Toxicity of Sarcandra Glabra Essential Oils on Tumor-bearing Mice Treated by Cytoxan / 浙江中医药大学学报

[Objective] To study synergistic effect and decreasing toxicity of Sarcandra Glabra essential oils (SGE) on H22 and S180 bearing mice treated with cytoxan (CTX).[Method] Transplanting H22 and S180 mice tumor models were established to observe the tumor inhibiting rate when SGE was used in combination with CTX by two different routes of administration.While the inhibition effects of SGE in decreasing blood platelet,leukocyte and hemoglobin induced by CTX were also detected.[Result] SGE has anti-tumor and synergy effect when combined with CTX on H22 and S180 bearing mice.The H22 and S180 tumor inhibiting rates of CTX (10mg?kg-1 or 15mg?kg-1) combined with SGE (36.6mg?kg-1) by iv.were increased compared with using CTX alone (P

Determination of Imperatorin and Isoimperatorin in Angelicae Dahuricae from Different Sources by HPLC / 浙江中医药大学学报

[Objective] To determine the contents of imperatorin and isoimperation in Angelicae dahuricae from different sources by HPLC,and prove some scientific evidence for processing standardization and quality control of Angelicae dahuricae decoction pieces.[Methods] An HPLC method was established.XB-C18 column was used,with mixture of acetonitrile and water in a gradient mode.The flow rate was 1.0mL?min-1.[Result] The calibration curve of imperatorin was in good linear over the range of 0.0552～0.276?g with the average assay recovery 99.5%.The calibration curve of isoimperatorin was linear over the range of 0.048～0.24?g with the average assay recovery 98.6% (RSD=1.7%).The contents of imperatorin from different sources in Angelicae dahuricae were between 0.046%～0.145%,isoimperatorin was between 0.038%～0.087%,the total was between 0.084%～0.232%.[Conclusion] This method was proved to be rapid,reproducible and accurate.It is suitable for the quality control of Angelilae dahuricae and its preparation.

The Research of Multi-factors Combination to Induce Lung-Yang Deficiency Rats / 浙江中医药大学学报

[Objective]We established a model of Lung-Yang Deficiency.[Methods]The factors such as smoking(exogenous evil effect on lung),swimming in ice water(cold body and exhaustion),swimming in common water(exhaustion),and taking the methimazole ice solution(immune suppression and inhale cold)were adopted to establish this Lung-Yang Deficiency model.SD rats were treated for 35 days,and their physical signs(diet changes,drinking and other typical symptoms)were observed.The hemorheological parameters of the treated animals were measured.[Results]At the later period,rats in the Lung-Yang Deficiency model showed several symptoms,such as wheeze,breathlessness,meanwhile,accompanied with dullness,drinking less water,chill.Compared with the normal group,the body weight and the back temperature of the treated rats were decreased significantly.The whole blood viscosity(WBV)and whole blood reduced viscosity(WBRV)on the low shear rate were significantly increased from the normal.So was found the rigidity finger,deformed finger and electrophoresis finger of RBC parameters.[Conclusion]This model is fit to the clinical investigation of Lung-Yang Deficiency,resulting in that our method for model establishment is feasible.

Effects of Different Fractions from Cortex Eucommiae on Oxidation Resistance and Anti-despondent of Extradiol-induced Kidney-yang Deficiency Mice / 浙江中医药大学学报

[Objective] To observe the effects of different fractions from Cortex Eucommiae on oxidation resistance and anti-despondent of Extradiol-induced kidney-yang deficiency mice,and understand the modern scientific connotation of the properties theories about sweet-flavor,warm-nature,meridian tropism in liver and kidney.[Methods] Healthy adult male ICR mice were divided into six groups randomly:normal control group,model control group,positive control group(Guifudihuang),three groups of Cortex Eucommiae(water fraction,n-butanol fraction,and acetoacetate fraction).Kidney-yang deficiency mice model was established with intraperitoneal injection of estradiol benzoate.The determined indexes included oxidation resistance indexes(activity of SOD,GSH-Px,CAT,content of MDA),anti-despondent indexes(tail suspension,activity of MAO),and anti-fatigue ability.[Results] Oxidation resistance indexes:Three fractions of Cortex Eucommiae all could decrease content of MDA,enhance activeness of SOD.The water and n-butanol fraction could enhance activeness of CAT.The water and acetoacetate fraction could enhance activeness of GSH-Px.Anti-despondent indexes:The acetoacetate fraction of Cortex Eucommiae could lengthen the activity time in tail suspension test,and three fractions of Cortex Eucommiae all could decrease activeness of MAO.Three fractions of Cortex Eucommiae all could lengthen the running distance and time.[Conclusion] Cortex Eucommiae could improve aging and depression caused by kidney-yang deficiency.Oxidation resistance and anti-despondent effects could be an important part of the main effective groups about sweet-flavor,warm-nature,meridian tropism in liver and kidney.The effects of water extract and n-butanol extract were closer and stronger,which could be the main substance basis of common effective groups.

Evaluation to Hypertension Model of Hyperaction of Liver Yang / 浙江中医药大学学报

In order to better serve disease experimental research and reach forecasted combination of disease and symptoms,it sums up the establishment of hypertension model of hyperaction of liver Yang,and makes exploration and induction to its evaluation index.

Preparation Technical Research of HP-?-CD Containing Ezhu Oil / 浙江中医药大学学报

[Objective] To study the best skill of HP-?-CD containing Ezhu oil.[Method] Take Ezhu oil containting rate as appraisal index,use orthogonal testing method for technical selection.[Result] Apply water solution mix for inclusion,the gotten best technical conditions:under 60 ℃,Ezhu oil:HP-?-CD=1:30,the regulating rotating speed 600 r/min/5 h;the total score of inclusion rate and recepting rate was 90.97%.[Conclusion] The preparation technical impoves stability of Ezhu oil,with better optimization,and offering realiable theoretic foundation for industrial production.

Effect of HQSM on PGI_2/TXA_2 in Blood Plasma of Myocardial Ischemia Rats with Pituitrin / 浙江中医药大学学报

[Objective] To observe the effect of HQSM on PGI_2/TXA_2 in blood plasma of acute myocardial ischemia rats caused by injecting pituitrin. [Methods] 84 SD rats were divided into seven groups at random: normal control group,model control group,three HQSM groups(210mg/kg,150mg/kg,105mg/kg),Huangqi Shengmaiyin and DI’ao Xinxuekang. Intragastric administration was adopted once a day for 30 days.Acute myocardial ischemia model rat was established by injecting pituitrin from tail vein 1h after the last administration, and blood plasma was separated to be determined the activity of 6-Keto-PGF_ 1? and TXB_2, which is the metabolite of PGI_2 and TXA_2 in blood plasma of SD rats. [Results] Having been injected pituitrin,the activity of TXB2 upgraded while 6-Keto-PGF_ 1? /TXB_2 decreased(P

Effect of HQSM on CK, LDH and AST in Serum of Myocardial Ischemia Rats / 浙江中医药大学学报

[Objective] To observe effects of HQSM on CK, LDH and AST activity in serum of acute myocardial ischemia rats caused by injecting pituitrin.[Method] SD rats were divided into several groups at random: normal control group,model control group, three HQSM groups(210mg/kg,150mg/kg,105mg/kg), positive control group(Huangqi Shengmaiyin and Diao Xinxuekang).The rats were treated with respective drugs by ig for 30 days, and pituitrin was injected respectively by caudal vein 1h after administering at the 29th and 30th days.Blood serum was taken out to determinate the activity of CK, LDH and AST at 1h and 24h after the last injection. [Result] The activity of LDH and AST in serum increased at 1h after injecting pituitrin, the activity of CK in serum increased,meanwhile LDH and AST was still higher than normal control group at 24h. HQSM could decrease the activity of CK and AST(P

Study on microwave-assisted extraction technique of flavonoid from Ophiopogon japonicus / 中华中医药杂志

Objective:To study microwave-assisted extraction technique of flavonoids from Ophiopogon japonicus. Methods:The effect of the parameters,i.e.ratio of solid to liquid,microwave treatment time,microwave power and volume ratio of ethanol on extraction amount of flavonoid were analyzed by single factor experiment.Results:Optimum processing data were determined as follows:90.0% ethanol as extracting solvent,stock ratio 1:14(g/ml) ,microwave extracting for 4 minutes at the power of MED. The extraction rate was 39.7% higher than that of non-microwave's.Conclusion:Extraction of flavonoids by microwave from Ophiopogon japonicus is an eficient method.

Effects of different extracts from Hippocampus japonicus on kidney-yang deficiency mice induced by extradiol / 中草药

Objective To observe effects of four extracts (water, n-butanol, acetoacetate and petro-leum benzine extracts) from Hippocampus japonicus on kidney-yang deficiency mice induced by extradiol, and reveal the modern scientific connotation about sweet-flavor, warm-nature, meridian tropism in liver and kidney. Methods Kidney-yang deficiency mice was induced by intraperitoneal injection of estradiol benzoate. Effects of four extracts from H. japonicus were observed by measuring physical signs, rectal temperature, grip strength, swimming time, autonomic mobility, creatinine (Cr) and urea (UR) level in serum, haematological indexes, testis index and seminal vesicle index. Results All the four extracts from H. japonicus could markedly improve physical signs and increase seminal vesicle index, RBC, Hb and PLT in blood, and decrease UR level in serum of kidney-yang deficiency mice, water extract and petoleum benzine extract could increase rectal temperature, grip strength, swimming time, autonomic mobility, testis index, WBC, lymphocyte (LY), monocyte (MO), and neutrophilic granulocyte (NE), n-butanol extract could increase autonomic mobility and NE, acetoacetate extract could enhance grip strength and prolong swimming time. Conclusion All above effects of extracts from H. japonicus could be as the basic of improving kidney-yang deficiency, could be an important part of the modern scientific connotation about sweet-flavor, warm-nature, meridian tropism in liver and kidney. Effects of water extract and petroleum ether extract were closer and stronger, which offer enlightenment for searching the common effective substance groups of H. japoniacs.